The anti-inflammatory effect of lycopene on neutrophil infiltration in the spleen of mice exposed to Lipopolysaccharide (LPS)
Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
Research Article
World Journal of Advanced Research and Reviews, 2024, 24(02), 246–252
Publication history:
Received 18 September 2024; revised on 31 October 2024; accepted on 02 November 2024
Abstract:
Introduction: Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria that can trigger inflammatory and oxidative stress responses. The purpose of this study is to observe the anti-inflammatory effect of lycopene in reducing neutrophil infiltration caused by LPS exposure in mice (Mus musculus).
Objective: This laboratory experimental study used 25 male mice aged 3 months with body weights of ± 25 g – 35 g, divided into five groups with five replicates each. C(-) is the control group: no LPS or lycopene was administered. C(+): received LPS 0.042 mg/kg without lycopene. P1: received LPS 0.042 mg/kg and lycopene 0.3 mg/kg. P2: received LPS 0.042 mg/kg and lycopene 0.6 mg/kg. P3: received LPS 0.042 mg/kg and lycopene 0.9 mg/kg. LPS was given intraperitoneally on days 1 and 8, and lycopene was administered daily for 14 days. The spleen is located near the liver. After the organ was removed, it was placed in 10% formalin buffer for HE staining, and data were analyzed using the Kruskall Wallis test followed by the Mann-Whitney test (p<0.05).
Results: LPS exposure significantly increased the number of neutrophils (p<0.05), and lycopene administration significantly reduced the number of neutrophils (p<0.05) to normal levels similar to the control (C-) at all doses/groups.
Conclusion: LPS exposure was proven to increase the number of neutrophils, and lycopene administration was able to reduce the number of neutrophils to normal levels in all dose groups.
Keywords:
Affordable Medicines; Lipopolysaccharide; Neutrophils; Mice; Lycopene
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